NMT作為生命科學(xué)底層核心技術(shù)，是建立活體創(chuàng)新科研平臺(tái)的*技術(shù)。2005年~2020年，NMT已扎根中國(guó)15年。2020年，中國(guó)NMT銷往瑞士蘇黎世大學(xué)，正式打開(kāi)歐洲市場(chǎng)。

研究使用平臺(tái)：NMT中藥材生長(zhǎng)生理創(chuàng)新科研平臺(tái)

期刊：Chemosphere

主題：生物炭介導(dǎo)微生物群落極其代謝特征

標(biāo)題：Biochar mediates microbial communities and their metabolic characteristics under continuous monoculture

影響因子：5.108

檢測(cè)指標(biāo)：NO₃^-、NH₄⁺、Na⁺、Ca²⁺、H⁺流速

檢測(cè)樣品：太子參

NO₃^-流實(shí)驗(yàn)處理方法：

0％、1％、3％（w/v）生物炭處理12小時(shí)

NO₃^-流實(shí)驗(yàn)測(cè)試液成份：
0.1mM NaCl，0.1mM CaCl₂，0.1 mM NH₄NO₃，pH 6.0

作者：福建農(nóng)林大學(xué)林文雄、林生、吳紅淼

中文摘要（谷歌機(jī)翻）

生物炭改良劑已被廣泛用于改善植物性能并抑制單一栽培系統(tǒng)中的病害。然而，很少有研究集中在再植疾病的基本控制機(jī)制上。

在這項(xiàng)研究中，我們?cè)u(píng)估了生物炭在連續(xù)單培養(yǎng)系統(tǒng)中對(duì)pseudo菜植物生長(zhǎng)，根際土壤微生物群落以及微生物生理特性的影響。我們發(fā)現(xiàn)生物炭的添加對(duì)假單胞菌組織培養(yǎng)的生理參數(shù)影響不大，但在不同連續(xù)單培養(yǎng)年的根際土壤中能顯著介導(dǎo)微生物的豐度，從而導(dǎo)致病原性尖孢鐮刀菌，Talaromyces helicus和科薩科尼亞糖精。

此外，生物炭改良劑對(duì)有益細(xì)菌的生長(zhǎng)具有不利影響，例如，伯克霍爾德氏菌，綠假單胞菌和短小芽孢桿菌。代謝組學(xué)分析表明，生物炭在抑制菌絲體生長(zhǎng)和減輕植物的毒力的同時(shí)，顯著影響了尖孢鐮刀菌的代謝過(guò)程。

總而言之，這項(xiàng)研究詳細(xì)說(shuō)明了生物炭刺激的根際細(xì)菌和真菌的豐度和代謝變化，病原體含量減少以及植物生長(zhǎng)環(huán)境條件改善的潛在機(jī)制。需要進(jìn)一步的研究來(lái)評(píng)估生物炭在長(zhǎng)期現(xiàn)場(chǎng)試驗(yàn)中的作用。

Steady state measurements of net fluxes in the meristematic zone of R. pseudostellariae under biochar treatment. CK, 1% biochar and 3% biochar represent the control with no biochar, 1% biochar and 3% biochar (w/v) added to the tissue culture media of R. psedostellariae for 12 h each, respectively. Data are mean ± standard deviation (n ¼ 5).

聯(lián)盟推薦抗疫產(chǎn)品之一

英文摘要

Biochar amendment has been extensively used to improve plant performance and suppress disease in monoculture systems; however, few studies have focused on the underlying control mechanisms of replanting disease.

In this study, we assessed the effects of biochar application on Radix pseudostellariae plant growth, rhizosphere soil microbial communities, and the physiological properties of microorganisms in a consecutive monoculture system. We found that biochar addition had little impact on the physiological parameters of tissue cultures of R. pseudostellaria but did significantly mediate microbial abundance in the rhizosphere soil of different consecutive monoculture years, leading to decreases in the abundance of pathogenic Fusarium oxysporum, Talaromyces helicus, and Kosakonia sacchari.

Furthermore, biochar amendment had negative effects on the growth of beneficial bacteria, such as Burkholderia ambifaria, Pseudomonas chlororaphis, and Bacillus pumilus. Metabolomic analysis indicated that biochar significantly influenced the metabolic processes of F. oxysporum while inhibiting the mycelial growth and abating the virulence on plants.

In summary, this study details the potential mechanisms responsible for the biochar-stimulated changes in the abundances and metabolism of rhizosphere bacteria and fungi, decreases in the contents of pathogens, and therefore improvements in the environmental conditions for plants growth. Further research is needed to evaluate the effects of biochar in long-term field trials.